Enantiomeric Separation of Underivatized Aliphatic β-Amino Alcohols by Ligand-exchange Chromatography usingN-n-Dodecyl-(1R,2S)-norephedrine as a Coating Reagent for Reversed-phase Column

1998 ◽  
Vol 21 (10) ◽  
pp. 561-564 ◽  
Author(s):  
Shigeo Yamazaki ◽  
Katsunori Saito ◽  
Takenori Tanimura
Keyword(s):  
Ligand Exchange ◽  
Amino Alcohols ◽  
Enantiomeric Separation ◽  
Reversed Phase ◽  
Exchange Chromatography ◽  
Ligand Exchange Chromatography ◽  
Phase Column ◽  
Reversed Phase Column

Ligand exchange chromatography of amino alcohols. Use of Schiff bases in enantiomer resolution

1984 ◽  
Vol 106 (25) ◽  
pp. 7729-7734 ◽  
Author(s):  
Loren R. Gelber ◽  
Barry L. Karger ◽  
John L. Neumeyer ◽  
Binyamin Feibush
Keyword(s):  
Schiff Bases ◽  
Ligand Exchange ◽  
Amino Alcohols ◽  
Exchange Chromatography ◽  
Ligand Exchange Chromatography ◽  
Enantiomer Resolution

Improved separation of C12-C22 fatty acid phenacyl esters by reversed phase column liquid chromatography

1986 ◽  
Vol 51 (12) ◽  
pp. 2722-2726 ◽  
Author(s):  
Tomáš Haniš ◽  
Miroslav Smrž ◽  
Pavel Klír ◽  
Karel Macek ◽  
Zdeněk Deyl
Keyword(s):  
Volume Concentration ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Isocratic Elution ◽  
Water Gradient ◽  
Phenacyl Esters ◽  
Acetonitrile Concentration ◽  
Phase Column ◽  
Reversed Phase Column ◽  
Critical Pairs

Phenacyl esters of C12-C22 fatty acids were separated on Separon SGX C18 column, using a gradient elution with methanol-acetonitrile-water. The proposed gradient showed better resolution of the critical pairs C18:3-C14:0, C16:1-C20:4, and C16:0-C18:1 than the gradient elution with methanol-water or acetonitrile-water, or than the isocratic elution with methanol-acetonitrile-water. The optimum volume concentration (83%) of the sum of both methanol and acetonitrile was maintained constant for 35 min; in this period the acetonitrile concentration decreased linearly from the initial 42-60% to 0% while the methanol concentration increased from the initial 41-23% to 83% at the same rate. After 35 min the elution was completed with a methanol-water gradient. The whole analysis can be performed within 63 min at a flow rate 1 ml/min.

4-Nitrophenol in 4-nitrophenyl phosphate, a substrate for alkaline phosphatase, as measured by paired-ion high-performance liquid chromatography.

1977 ◽  
Vol 23 (12) ◽  
pp. 2288-2291 ◽  
Author(s):  
P H Culbreth ◽  
I W Duncan ◽  
C A Burtis
Keyword(s):  
Alkaline Phosphatase ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Flow Rate ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Nitrophenyl Phosphate ◽  
Phase Column ◽  
Reversed Phase Column

Abstract We used paired-ion high-performance liquid chromatography to determine the 4-nitrophenol content of 4-nitrophenyl phosphate, a substrate for alkaline phosphatase analysis. This was done on a reversed-phase column with a mobile phase of methanol/water, 45/55 by vol, containing 3 ml of tetrabutylammonium phosphate reagent per 200 ml of solvent. At a flow rate of 1 ml/min, 4-nitrophenol was eluted at 9 min and monitored at 404 nm; 4-nitrophenyl phosphate was eluted at 5 min and could be monitored at 311 nm. Samples of 4-nitrophenyl phosphate obtained from several sources contained 0.3 to 7.8 mole of 4-nitrophenol per mole of 4-nitrophenyl phosphate.

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